Blastn Genbank index error

Biomedical and Bioinformatics Research
1

Hi, I am trying to blast to the shared genbank database but am getting the following error. Perhaps the database was not indexed properly?

Error:
Inactive Modules:

  1. py-cython

Due to MODULEPATH changes, the following have been reloaded:

  1. bzip2/1.0.8

The following have been reloaded with a version change:

  1. gcc/11.3.0 => gcc/9.2.0 4) perl/5.36.0 => perl/5.30.0
  2. htslib/1.17 => htslib/1.10.2 5) python/3.11.3 => python/3.7.6
  3. openblas/0.3.21 => openblas/0.3.7 6) zlib/1.2.12 => zlib/18.0.4

BLAST Database error: No alias or index file found for nucleotide database [/project/biodb/genbank/2023-03-01/FASTA/nt] in search path [/scratch1/mruggeri/Aagar_denovo/Ref/subset10_cdh_alltags::]

Job script:
#!/bin/bash

#SBATCH --ntasks=1

#SBATCH --cpus-per-task=16

#SBATCH --mem-per-cpu=2G

#SBATCH --time=06:00:00

#SBATCH --error=%x_%j.err

#SBATCH --output=%x_%j.out

#SBATCH --mail-type=END

#SBATCH --mail-user=mruggeri@usc.edu

module load gcc/9.2.0

module load blast-plus/2.11.0

blastn -query subset10_cdh_alltags.fasta -db /project/biodb/genbank/2023-03-01/FASTA/nt -evalue 0.0001 -num_threads 16 -num_descriptions 5 -num_alignments 5 -out subset10_cdh_alltags.br